Fig 1: 3CLpro cleavage of CDH6 and CDH12.A, sequence alignment of selected cadherin proteins; CDH6 contains five cadherin domains (54–608), “.” indicates the same amino acid compared with CDH6, “↓” indicates the cleavage site. B, CDH6 and CDH12 cleavage analysis. Lane M: protein marker, lane 1: 3CLpro; lane 2: CDH6; lane 3: 3CLpro + CDH6, incubation at 37 °C for 4 h, “←” indicates the cleaved CDH6; lane 4: 3CLpro; lane 5: CDH12; lane 6: 3CLpro + CDH12, incubation at 37 °C for 4 h, “←” indicates the cleaved CDH12. 3CLpro, 3C-like protease.
Fig 2: CDH6 and CDH12 cleavage sites confirmation.A, lane M: protein marker, lane 1: CDH6, lane 2: CDH6 + PNGase F, lane 3: CDH6 + PNGase F + 3CLpro, lane 4: CDH12, lane 5: CDH12 + PNGase F, lane 6: CDH12 + PNGase F + 3CLpro, “←” indicates the cleaved N-terminal fragments of CDH6 and CDH12. B and C, mass spectrometry analysis; the calculated mass of CDH6 (G54-Q203) is 16974.8 Da, and the calculated mass of CDH12 (G55-Q204) is 16721.6 Da. 3CLpro, 3C-like protease; CDH6, cadherin-6; PNGase F, peptide PNGase F.
Supplier Page from Sino Biological, Inc. for Human Cadherin-12 / CDH12 Protein (His Tag)